Bench to Pilot
Moving from 2L bioreactor to 100–500L pilot scale is the most common failure point in precision fermentation. kLa drops 3–10× and substrate gradients appear for the first time. Fermvyne pre-identifies the DO and feed rate corrections needed before the pilot run happens.
- Predict kLa at pilot scale from vessel geometry
- Identify feed rate ceiling before overflow onset
- Generate pilot-specific agitation cascade setpoints
- Compare DO profiles: optimistic vs risk-weighted
Bench to Pilot Details
Pilot to Commercial
At 2,000–20,000L, hydrostatic pressure effects, longer mixing times, and impeller selection become dominant variables. A 30% titer drop at commercial scale can represent $200K+ per run. Fermvyne's commercial-scale module accounts for pressure depth correction and shear sensitivity.
- Hydrostatic depth-pressure correction for kLa
- Mixing time estimation for substrate homogeneity
- Shear sensitivity flagging for sensitive organisms
- Multi-impeller configuration comparison
Pilot to Commercial Details
Strain Optimization
When evaluating multiple strain candidates from your engineering pipeline, metabolic modeling predicts how each strain's flux phenotype will perform at scale — before you invest pilot run costs in candidates likely to fail due to overflow or oxygen sensitivity at higher density.
- Compare up to 5 strain variants per simulation job
- Identify overflow-prone phenotypes at high density
- Predict optimal induction OD for recombinant proteins
- Screen for DO sensitivity before pilot selection
Strain Optimization Details