Worked examples, parameter selection guides, and methodology details. No sales language — written by scientists for scientists.
Agitation cascade setpoint selection for aerobic E. coli fed-batch culture based on vessel geometry and target OD600. Includes worked example for 500L Rushton-equipped bioreactor moving from 2L bench data. Covers minimum kLa requirements at each cell density stage, agitation ramp timing, and the DO-stat fallback protocol when kLa is insufficient at peak density.
How to calculate the maximum specific glucose uptake rate before overflow onset using bench-scale flux data. Includes derivation of the q_glc_max parameter from OD600, glucose consumption rate, and dissolved oxygen measurements. Provides a lookup table of overflow-onset feed rates by OD600 for three common E. coli BL21(DE3) derivatives.
Oxygen demand calculation during methanol induction in P. pastoris expression systems. Covers the stoichiometry of methanol oxidation, the kLa requirements during induction at three common expression densities (50, 100, 200 g DCW/L), and the transition protocol from glycerol batch to methanol feed that minimizes DO depletion during the feed switch.
FBA-based analysis of fumarate and malate accumulation in Fusarium venenatum under DO-limited conditions. Identifies the critical DO% below which TCA cycle imbalance drives organic acid accumulation. Includes scale-specific DO maintenance setpoints for 500L and 5,000L continuous culture and the relationship between specific growth rate and fumarate flux.
Depth-weighted pressure correction methodology for kLa estimation in commercial-scale vessels. Covers the derivation of the pressure correction factor from vessel height and liquid density, the resulting DO partial pressure gradient, and its effect on the apparent kLa measured by a mid-vessel DO probe vs the actual transfer rate at the base. Includes worked example for 10,000L STR at 3m liquid height.
The full text, equations, and worked examples are available in PDF format to Fermvyne users and early access registrants. Request access below.